Introducing transgalactosylation activity into a family 42 β-galactosidase

Transgalactosylation activity of ebg beta-galactosidase synthesizes allolactose from lactose.

ebg enzyme, the second beta-galactosidase of Escherichia coli, does not normally convert lactose into an inducer of the lac operon. We previously reported the existence of a mutant ebg enzyme that does make such an inducer in vivo (Rolseth et al., J. Bacteriol. 142:1036-1039, 1980). Here I report that the mutant enzyme makes inducer from lactose in vitro and that the inducer is allolactose. Al...

Application of Transgalactosylation Activity of β-Galactosidase from Kluyveromyces lactis for the Synthesis of Ascorbic Acid Galactoside

In view of a commonly known beneficial role and low stability of ascorbic acid, many efforts are constantly undertaken to produce its improved derivatives. This paper presents results on the synthesis of ascorbic acid galactoside using transgalactosylation properties of β-galactosidase from Kluyveromyces lactis and lactose as a donor of galactosyl moiety. The purpose of this study was to determ...

Characterization of a Salt-Tolerant Family 42 b-Galactosidase from a Psychrophilic Antarctic Planococcus Isolate

Characterization of a salt-tolerant family 42 beta-galactosidase from a psychrophilic antarctic Planococcus isolate.

We isolated a gram-positive, halotolerant psychrophile from a hypersaline pond located on the McMurdo Ice Shelf in Antarctica. A phylogenetic analysis of the 16S rRNA gene sequence of this organism showed that it is a member of the genus Planococcus. This assignment is consistent with the morphology and physiological characteristics of the organism. A gene encoding a beta-galactosidase in this ...

Transgalactosylation products from melibose by the alpha-galactosidase of Absidia corymbifera.

such a galactosyl transfer reaction as well as the decomposition of raffinose in beet sugar production, they may also produce galactooligosaccharides. Mitutomi and OhtakaraX) reported the isolation and identification of six main oligosaccharides produced from raffinose by the action of the a-galactosidase from Pycnoporus cinnabarinus. In our preliminary experiments, however, the a-galactosidase...